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系統識別號 U0002-3108202012143000
中文論文名稱 製備溫感性抗菌水凝膠以應用在創傷敷材
英文論文名稱 Fabrication of thermo-responsive antibacterial hydrogels for wound dressing
校院名稱 淡江大學
系所名稱(中) 化學工程與材料工程學系碩士班
系所名稱(英) Department of Chemical and Materials Engineering
學年度 108
學期 2
出版年 109
研究生中文姓名 邱詠翰
研究生英文姓名 Yung-Han Chiu
學號 607400016
學位類別 碩士
語文別 中文
口試日期 2020-07-13
論文頁數 120頁
口試委員 指導教授-董崇民
委員-蔡敏郎
委員-黃意真
中文關鍵字 創傷敷材  水凝膠  溫感性  氮-異丙基丙烯醯胺  幾丁聚醣 
英文關鍵字 Wound dressing  Hydrogel  Thermosensitive  N-isopropylacrylamide  Chitosan 
學科別分類
中文摘要 本研究為利用紫外光起始自由基聚合法合成具溫度敏感性的抗菌水凝膠,以氮-異丙基丙烯醯胺 (N-isopropylacrylamide,NIPAAm,NP)為主體,添加親水性良好的2-丙烯醯胺-2-甲基丙磺酸 (2-acrylamido-2-methylpropane sulfonic acid,AMPS)、2-丙烯酸羥乙酯 (2-Hydroxyethyl acrylate,HEA)以及幾丁聚醣 (Chitosan,CS),並加入N,N'-亞甲基雙丙烯醯胺 (N,N'-methylenebisacrylamide,MBA)和2,2-二乙氧基苯乙酮 (2,2-diethoxyacetophenone,DEAP)做為交聯劑與光起始劑,形成半互穿型的網狀水凝膠,利用FTIR對其化學結構進行分析,確定水凝膠有成功合成,並用SEM觀察水凝膠之表面型態,接著進行拉伸機械性質、熱性質、膨潤測試、藥物包覆/釋放、生物相容性、抗菌等一系列測試,對水凝膠進行評估,根據拉伸測試結果可以知道交聯劑及NP含量的提升都可以增加抗張強度及破壞伸長率,膨潤的情況下,抗張強度與拉伸率最高可以達到67.5 kPa和55.6%;熱性質方面,隨著NP相對含量的提升,玻璃轉移溫度可以從N9A1-1.0的116 oC提升至N99A1-1.0的125 oC,有助於增強水凝膠的熱穩定性;膨潤率的結果顯示出水凝膠的平衡膨潤率隨著交聯劑和NP的相對含量提升而下降;水凝膠對於鹽酸四環素 (Tetracycline hydrochloride,TC)的包覆與釋放結果可以發現,其鹽酸四環素的包覆率會隨著NP的含量提高而下降,藥物釋放行為不會受到pH值所影響且經由動力學的描述皆屬於異常擴散 (Anomalous transport,n介於0.5到1.0之間);透過人類表皮纖維母細胞HS 68所進行的生物相容性結果可以知道水凝膠對於細胞是沒有毒性的;大腸桿菌的抗菌實驗顯示出水凝膠具有抗菌性。
英文摘要 In this study, N-isopropylacrylamide, 2-acrylamido-2-methylpropane sulfonic acid and 2-Hydroxyethyl acrylate were polymerized by UV-initiated free radical polymerization in the presence of chitosan to prepare the semi-interpenetrating hydrogels with thermo-responsive and antibacterial properties. N,N'-methylenebisacrylamide and 2,2-diethoxyacetophenone were applied as crosslinker and photo-initator, respectively. Chemical structure and surface morphology of hydrogels were characterized by respective FTIR and SEM. Furthermore, a series of tests iucluding tensile mechanical properties, thermal properties, swelling, drug loading/release, biocompatibility, antibacterial properties were carried out to evaluate the prepared hydrogels. The results showed that increasing the contents of NP and crosslinking agent would enhance the tensile mechanical properties in which the tensile strength and enlongation at break could reach 67.5 kPa and 55.6%, respectively, under swelling state. Moreover, glass transition temperature (Tg) increased from 116 oC for the N9A1-1.0 to 125 oC at the NP content was increased. The results also showed that the increases of crosslinker and NP content would reduce the equilibrium swelling ratio. The loading amount of tetracycline hydrochloride in the hydrogels decreased with the increasing NP content and the drug-release behavior would not be influenced by pH value. The drug-release behavior could be described by an empirical equation of non-fickian diffusion. In vitro studies showed that hydrogels were non-toxic and they could promote cell growth of human foreskin fibroblast (HS-68). The antibacterial test against E. coli showed that these hydrogels had antibacterial properties.
論文目次 目錄
目錄 V
圖目錄 VIII
表目錄 XIII
第一章 緒論 1
1.1前言 1
1.2研究動機 2
第二章 文獻回顧 3
2.1傷口護理&傷口敷料 3
2.1.1人體皮膚構造 3
2.1.2傷口形成&癒合機制 4
2.1.3傷口敷料之發展 8
2.1.4傷口敷料之種類與選擇 9
2.2水凝膠 12
2.2.1水凝膠簡介 12
2.2.2水凝膠作為傷口敷料之介紹 14
2.3幾丁聚醣 15
2.3.1幾丁質與幾丁聚醣簡介 15
2.3.2幾丁聚醣作為傷口敷料之潛力 17
2.4環境敏感型高分子 20
2.4.1環境敏感型高分子簡介 20
2.4.2聚(N-異丙基丙烯醯胺)與其共聚合體 25
2.5光起始聚合反應 27
第三章 實驗 29
3.1實驗藥品 29
3.2實驗儀器 32
3.3實驗步驟 36
3.3.1幾丁聚醣純化 36
3.3.2 NIPAAm單體純化 36
3.3.3水凝膠製備 36
3.4結構分析 39
3.4.1幾丁聚醣去乙醯度測量 (H-NMR、UV-VIS、電位滴定) 39
3.4.2幾丁聚醣黏度平均分子量測定 (毛細管黏度計) 40
3.4.3官能基分析 (FTIR) 42
3.4.4水凝膠型態分析 (SEM) 42
3.5性質測試 43
3.5.1拉伸機械性質測試 43
3.5.2熱性質分析 43
3.5.3膨潤率 & 膨潤動力學 44
3.5.4膨潤/去膨潤響應 44
3.5.5藥物包覆&釋放 45
3.5.6生物相容性測試 46
3.5.7抗菌測試 48
第四章 結果與討論 49
4.1幾丁聚醣結構分析 49
4.1.1去乙醯度 (Degree of deacetylation,DDA%) 49
4.1.2黏度平均分子量 54
4.1.3幾丁聚醣結構鑑定 (FTIR) 56
4.2水凝膠結構分析 57
4.2.1結構鑑定 (FTIR) 57
4.2.2水凝膠型態分析 (SEM) 59
4.3水凝膠性質分析 62
4.3.1拉伸機械性質 62
4.3.2熱重損失分析 (TGA) 63
4.3.3玻璃轉移溫度 (DSC) 67
4.3.4膨潤特性 69
4.3.5膨潤動力 74
4.3.6膨潤/去膨潤響應 79
4.3.7藥物包覆&釋放 80
4.3.8釋放動力學 84
4.3.9生物相容性測試 90
4.3.10抗菌測試 91
第五章 結論 93
5.1 幾丁聚醣性質測試 93
5.2水凝膠結構分析 93
5.3水凝膠性質測試 93
第六章 建議事項 95
第七章 文獻回顧 96
第八章 附錄 105
8.1膨潤動力學 (Zero order & Higuchi equation) 105
8.2藥物包覆後之表面形態 118
8.3背景吸光值對於生物相容性結果的影響 119


圖目錄
圖2-1皮膚構造圖 3
圖2-2正常傷口和慢性傷口的癒合過程 4
圖2-3傷口修復各階段 6
圖2-4傷口癒合各階段細胞作用示意圖 7
圖2-5傷口敷料之簡易分類 8
圖2-6合成敷料的種類 11
圖2-7理想創傷敷材的標準 11
圖2-8物理交聯葡萄糖響應水凝膠示意圖 13
圖2-9化學交聯水凝膠示意圖 13
圖2-10幾丁質結構 16
圖2-11纖維素結構 16
圖2-12不同排列方式的幾丁質 16
圖2-13幾丁聚醣結構 17
圖2-14幾丁聚醣之生物應用 19
圖2-15幾丁聚醣之加工型態 19
圖2-16溫度敏感型高分子之溶解度相變化圖 20
圖2-17 PNIPAAm & PDEAAm結構圖 21
圖2-18 PNIPAAm溫感性細胞吸脫附作用示意圖 22
圖2-19 (a)PAA、(b)PMAA、(c)PDMAEMA、(d)PDEAEMA結構式 23
圖2-20紫外光誘導濕潤性變化 24
圖2-21新型二硒化物交聯水凝膠之藥物釋放示意圖 26
圖2-22電磁波譜 28
圖3-1經紫外光硬化之水凝膠 37
圖4-1幾丁聚醣H-NMR光譜圖 49
圖4-2不同濃度之N-乙醯基-D-葡萄糖胺一次微分吸光圖 50
圖4-3 N-乙醯基-D-葡萄糖胺一次微分吸光值對濃度之檢量線 51
圖4-4幾丁聚醣溶液之導電度與pH值隨加入的NaOH體積變化曲線 52
圖4-5幾丁聚醣溶液之pH值變化曲線一次微分 53
圖4-6幾丁聚醣溶液在不同濃度下之還原黏度 (ηred)與固定黏度 (ηinh) 55
圖4-7幾丁聚醣紅外線吸收光譜圖 56
圖4-8(a)NIPAAm、(b)AMPS、(c)2-HEA、(d)N9A1-1.0、(d)N29A1-1.0 58
圖4-9 N9A1-0.5水凝膠之掃描式電子顯微鏡圖 60
圖4-10 N9A1-1.0水凝膠之掃描式電子顯微鏡圖 60
圖4-11 N29A1-1.0水凝膠之掃描式電子顯微鏡圖 61
圖4-12不同組成水凝膠在完全膨潤的情況下之抗張強度(kPa)與伸長率(%) 62
圖4-13不同交聯劑比例之水凝膠的熱重損失圖 65
圖4-14不同交聯劑比例之水凝膠的熱重損失微分圖 65
圖4-15不同NP/AMPS比例之水凝膠的熱重損失圖 66
圖4-16不同NP/AMPS比例之水凝膠的熱重損失微分圖 66
圖4-17不同交聯劑含量之水凝膠的DSC一次升溫曲線圖 68
圖4-18不同NP/AMPS比例之水凝膠的DSC一次升溫曲線圖 68
圖4-19不同組成的水凝膠在25 oC、pH 5.5緩衝溶液中膨潤率隨時間之變化 70
圖4-20不同組成的水凝膠在25 oC、pH 7.4緩衝溶液中膨潤率隨時間之變化 71
圖4-21不同組成的水凝膠在37 oC、pH 5.5緩衝溶液中膨潤率隨時間之變化 71
圖4-22不同組成的水凝膠在37 oC、pH 7.4緩衝溶液中膨潤率隨時間之變化 72
圖4-23不同組成的水凝膠在不同溫度及不同pH值緩衝液中的平衡膨潤率 73
圖4-24水凝膠在pH 5.5、25 oC緩衝溶液下膨潤率ln(SRt/SReq)對lnt作圖 75
圖4-25水凝膠在pH 7.4、25 oC緩衝溶液下膨潤率ln(SRt/SReq)對lnt作圖 76
圖4-26水凝膠在pH 5.5、37 oC緩衝溶液下膨潤率ln(SRt/SReq)對lnt作圖 77
圖4-27水凝膠在pH 7.4、37 oC緩衝溶液下膨潤率ln(SRt/SReq)對lnt作圖 78
圖4-28不同組成水凝膠於25 oC及37 oC水溶液下交替膨潤/去膨潤響應行為 79
圖4-29鹽酸四環素檢量線 (D.I.W,274 nm) 80
圖4-30鹽酸四環素檢量線 (PBS,274 nm) 82
圖4-31不同組成的水凝膠在37 oC、pH 5.5緩衝溶液中藥物累積釋放率隨時間之變化 83
圖4-32不同組成的水凝膠在37 oC、pH 7.4緩衝溶液中藥物累積釋放率隨時間之變化 83
圖4-33水凝膠在pH 5.5、37 oC緩衝溶液下藥物累積釋放率Mt/M0對t作圖 85
圖4-34水凝膠在pH 7.4、37 oC緩衝溶液下藥物累積釋放率Mt/M0對t作圖 85
圖4-35水凝膠在pH 5.5、37 oC緩衝溶液下藥物累積釋放率Mt/M0對t1/2作圖 86
圖4-36水凝膠在pH 7.4、37 oC緩衝溶液下藥物累積釋放率Mt/M0對t1/2作圖 87
圖4-37水凝膠在pH 5.5、37 oC緩衝溶液下藥物累積釋放率ln(Mt/Mmax)對lnt作圖 88
圖4-38水凝膠在pH 7.4、37 oC緩衝溶液下藥物累積釋放率ln(Mt/Mmax)對lnt作圖 89
圖4-39不同組成水凝膠對HS68細胞存活率之影響 90
圖4-40不同比例水凝膠對於大腸桿菌之抗菌活性百分比 92
圖8-1水凝膠在pH 5.5、25 oC緩衝溶液下膨潤率SRt對t作圖 (前60 min) 106
圖8-2水凝膠在pH 7.4、25 oC緩衝溶液下膨潤率SRt對t作圖 (前60 min) 106
圖8-3水凝膠在pH 5.5、37 oC緩衝溶液下膨潤率SRt對t作圖 (前60 min) 107
圖8-4水凝膠在pH 7.4、37 oC緩衝溶液下膨潤率SRt對t作圖 (前60 min) 108
圖8-5水凝膠在pH 5.5、25 oC緩衝溶液下膨潤率SRt對t1/2作圖 (前60 min) 109
圖8-6水凝膠在pH 7.4、25 oC緩衝溶液下膨潤率SRt對t1/2作圖 (前60 min) 109
圖8-7水凝膠在pH 5.5、37 oC緩衝溶液下膨潤率SRt對t1/2作圖 (前60 min) 110
圖8-8水凝膠在pH 7.4、37 oC緩衝溶液下膨潤率SRt對t1/2作圖 (前60 min) 111
圖8-9水凝膠在pH 5.5、25 oC緩衝溶液下膨潤率SRt對t作圖 (前120 min) 112
圖8-10水凝膠在pH 7.4、25 oC緩衝溶液下膨潤率SRt對t作圖 (前120 min) 112
圖8-11水凝膠在pH 5.5、37 oC緩衝溶液下膨潤率SRt對t作圖 (前120 min) 113
圖8-12水凝膠在pH 7.4、37 oC緩衝溶液下膨潤率SRt對t作圖 (前120 min) 114
圖8-13水凝膠在pH 5.5、25 oC緩衝溶液下膨潤率SRt對t1/2作圖 (前120 min) 115
圖8-14水凝膠在pH 7.4、25 oC緩衝溶液下膨潤率SRt對t1/2作圖 (前120 min) 115
圖8-15水凝膠在pH 5.5、37 oC緩衝溶液下膨潤率SRt對t1/2作圖 (前120 min) 116
圖8-16水凝膠在pH 7.4、37 oC緩衝溶液下膨潤率SRt對t1/2作圖 (前120 min) 117
圖8-17鹽酸四環素水凝膠之掃描式電子顯微鏡表面圖 (a) N9A1-0.5、 118
圖8-18 CCK-8測試中背景組在400~600 nm之光譜吸收圖 (背景組含CCK-8試劑與培養基) 120
圖8-19不同組成水凝膠對HS68細胞存活率之影響 (不扣除背景值之結果) 120



表目錄
表2-1市面上常見敷料之原料與優缺點 9
表2-2不同類型的傷口適合之傷口敷料 10
表2-3 UVA、UVB、UVC紫外光波長與強度統整 28
表3-1水凝膠各添加物之比例與重量 38
表3-2幾丁聚醣的黏度常數a和K與溶劑、溫度、去乙醯度之關係 41
表4-1不同方法所測定之幾丁聚醣去乙醯度統整表 53
表4-2不同濃度的幾丁聚醣樣品所測得之滯留時間與其黏度參數 54
表4-3各單體之官能基紅外線吸收峰位置整理 58
表4-4不同組成水凝膠在完全膨潤的情況下之抗張強度(kPa)與破壞伸長率(%) 63
表4-5不同組成水凝膠之起始重量損失溫度 (Td, 5%)、最大速率裂解溫度 (Tmax)以及炭焦殘餘量 (C.Y.) 64
表4-6不同比例水凝膠之一次升溫玻璃轉移溫度 (Tg) 67
表4-7不同組成的水凝膠在不同溫度及pH值緩衝液中的平衡膨潤率 (ESR, %) 72
表4-8不同組成的水凝膠在不同溫度下 (25與37oC)之平衡膨潤率差異百分比 (SRD,%) 73
表4-9不同水凝膠之膨潤動力學參數n、k、R2值 (pH 5.5、25 oC) 75
表4-10不同水凝膠之膨潤動力學參數n、k、R2值 (pH 7.4、25 oC) 76
表4-11不同水凝膠之膨潤動力學參數n、k、R2值 (pH 5.5、37 oC) 77
表4-12不同水凝膠之膨潤動力學參數n、k、R2值 (pH 7.4、37 oC) 78
表4-13不同組成之水凝膠於鹽酸四環素水溶液之包覆結果 (藥物濃度: 1 mg/ml,25 oC) 81
表4-14 不同水凝膠之Zero order equation釋放動力學參數kz、R2值 (37 oC) 86
表4-15不同水凝膠之Higuchi equation釋放動力學參數kH、R2值 (37 oC) 87
表4-16不同水凝膠之經驗方程式釋放動力學參數n、k、R2值 (pH 5.5、37 oC) 88
表4-17不同水凝膠之經驗方程式釋放動力學參數n、k、R2值 (pH 7.4、37 oC) 89
表4-18不同比例水凝膠對於大腸桿菌之抗菌活性與落菌形成單位 91
表8-1不同水凝膠之Zero order equation膨潤動力學參數kz、R2值 (25 oC,前60 min) 107
表8-2不同水凝膠之Zero order equation膨潤動力學參數kz、R2值 (37 oC,前60 min) 108
表8-3不同水凝膠之Higuchi equation膨潤動力學參數kH、R2值 (25 oC,前60 min) 110
表8-4不同水凝膠之Higuchi equation膨潤動力學參數kH、R2值 (37 oC,前60 min) 111
表8-5不同水凝膠之Zero order equation膨潤動力學參數kz、R2值 (25 oC,前120 min) 113
表8-6不同水凝膠之Zero order equation膨潤動力學參數kz、R2值 (37 oC,前120 min) 114
表8-7不同水凝膠之Higuchi equation膨潤動力學參數kH、R2值 (25 oC,前120 min) 116
表8-8不同水凝膠之Higuchi equation膨潤動力學參數kH、R2值 (37 oC,前120 min) 117

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