先前本實驗室研究指出，在低密度菌體發酵時，酵母菌酒精轉化率極高，但是在低密度表現良好的菌株未如預期在高密度菌體發酵有高的精轉化率。因此為了尋找在高密度條件下菌株發酵轉化率高的菌株，本實驗採用的篩菌方法為高密度菌體之連續液態篩選法，以圖找到在高密度發酵良好的菌株。

Previous studies in our laboratory have pointed out that the alcohol conversion rate of yeast is extremely high when low-density bacterial fermentation is performed, but strains that perform well at low density do not have high alcohol conversion rate as expected in high-density bacterial fermentation.
Therefore, in order to search for strains with high fermentation conversion rate under high-density conditions, the screening method adopted in this experiment is a continuous liquid screening method for high-density bacteria, in order to find a strain that ferments well at high density.

目錄
謝誌 ...............................................I
高效率發酵菌種篩選  ................................1
第一章	緒論  ..........................................1
1.1前言  ......................................1
1.2文獻回顧  ..................................1
1.3研究動機  ..................................1
第二章 材料與方法.....................................2
       2.1.1實驗器材藥品儀器 .........................2
     2.1.2藥品配方..................................3
     2.1.3菌株 .....................................3
       2.1.4實驗步驟................................. 3-5
       2.1.4-1 UV突變 ................................4
       2.1.4-2 高糖高酒精篩選..........................4
       2.1.4-3生長能力測試  ..........................4
       2.1.4-4 酒精發酵能力測試 .......................5
       2.1.4-5 葡萄糖能力測試..........................5
第三章  實驗結果.......................................6-10
       3.1菌株突變.....................................6
       3.2菌株篩選.....................................6
       3.3酒精發酵能力測試.............................6
       3.4葡萄糖能力測試...............................9
        附錄酵母菌生長能力測試100株和野生種比值.....10-27
第四章  討論...........................................28
參考文獻...............................................29

1.	Essen, L.O., Photolyases and cryptochromes: common mechanisms of DNA repair and light-driven signaling? Curr Opin Struct Biol, 2006. 16(1): p. 51-9.
2.	Alper, H., et al., Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production. Science, 2006. 314: p. 1565-1568.
3.	Lin, Y., et al., Factors affecting ethanol fermentation using Saccharomyces cerevisiae BY4742. Biomass and Bioenergy, 2012. 47: p. 395-401.
4.	Felix H. Lam,1,2 Adel Ghaderi,1 Gerald R. Fink,2* Gregory Stephanopoulos1*: Engineering alcohol tolerance in yeast, OCTOBER 2014 • VOL 346 ISSUE 6205 p71-73
5.	林建利碩士班論文