本論文以毛細管電泳(CE)技術分別建立測量幾丁聚醣的去乙醯化程度(DDA)和分子量(Mv)的校正曲線。在測量不同DDA(26%~95%)幾丁聚醣樣品於不同濃度(25 mM~1.6 M) 毛細管電泳緩衝溶液(tris-phosphate buffer, pH = 2.00)中的電泳遷移率(μ)時，我們發現，由CE所建構的μ對DDA(NMR測量值)校正曲線在樣品之高DDA範圍(55%~95%)和低DDA範圍(26%~ 55%)有不同的線性關係，而隨著緩衝溶液濃度提高，兩線性漸趨一致，在毛細管電泳緩衝溶液達1.5 M時，兩段DDA範圍(26%~95%)即呈一線性關係。另外，毛細管電泳圖的UV吸收峰(200 nm)訊號之半高寬(W1/2, DDA)代表樣品的DDA分布情形，我們發現將樣品進行依我們所設計的去乙醯化反應，再經乙醯化反應後，可得到較小分布但DDA不變的樣品。而毛細管電泳快速和微量分析的特性，更有利於我們探討幾丁質和幾丁聚醣的去乙醯化反應之動力學。
    我們也利用CE測量幾丁聚醣樣品的黏度，以Mark-Houwink equation計算其極限黏度分子量(Mv)，再測量不同Mv幾丁聚醣在毛細管凝膠電泳(CGE, 分離介質為0.3% PEO in 100 mM tris-phosphate buffer, pH = 2.00)中的電泳遷移率，建立Log(μ)對Mv的校正曲線。據此，我們探討了以過硫酸鉀(potassium persulfate, KPS)為主的化學裂解法，和以胃蛋白酶(pepsin)、脂解酶(lipase )、和胰蛋白酶(trypsin)為主的酵素消化法，對於幾丁聚醣分子量降解反應的影響。
     除了以CE分析幾丁聚醣降解後的多醣產物，我們也使用MALDI-TOF MS偵測分子量降解後的幾丁寡醣產物。根據MALDI所測得的質譜圖和數據，我們可以分析降解產物的單體組成，並比較不同降解方法所得產物的異同。

In this thesis, we used capillary electrophoresis (CE) technique to establish the respective calibration curves for the measurements of degree of deacetylation (DDA) and molecular weight (Mv) for chitosan samples. When measuring the electrophoretic mobility (μ) of chitosan samples with DDA values ranging from 26% to 95% in CE run buffers (tris-phosphate buffer, pH = 2.00) of various concentrations (25 mM~1.6M), we found that the μ-versus-DDA (NMR measured) calibration curves possess different linearity in the higher DDA region (55%~95%) and in the lower DDA region (26%~55%). Moreover, the linearity of the two regions gradually approach to each other with increasing buffer concentrations, and finally become one linear relation in the DDA range of 26%~95% when raising the CE buffer concentration up to 1.5 M. In addition, the UV absorption peak (at 200 nm) width at half maximum (W1/2, DDA) in the CE electropherogram enable us to obtain the DDA distribution in the chitosan samples. We found that chitosan with unchanged DDA but narrower DDA distribution can be generated by designed deacetylation and acetylation reactions. The high efficiency and microanalysis features of our CE based method also facilitate the kinetic studies of the deacetylation reactions for chitin and chitosan samples.
We also used CE to measure the viscosity of the chitosan samples so as to calculate the intrinsic-viscosity defined molecular weight (Mv) by the Mark-Houwink equation, and to establish the Log(μ)-versus-Mv calibration curve by measuring the electrophoretic mobility of chitosan samples with varied Mv’s in capillary gel electrophoresis (CGE, with separation medium of 0.3% PEO in 100 mM tris-phosphate buffer, pH = 2.00). By using the CGE technique we were able to study the Mv degradation reactions of chitosan by the chemical degradation method, which is mainly initiated by potassium persulfate (KPS), and by the enzymatic digestion approach, which is based on the biological enzymes such as pepsin, lipase, and trypsin. 
Besides CE, we also used MALDI-TOF mass to detect the chitooligosaccharide product of the chitosan degradation reaction. According to the MALDI-TOF mass spectrums and data, we can analyze and make a comparison among the monomer compositions of the chitooligosaccharide obtained from the abovementioned different fragmentation methods.

第一章 緒論	1
1.1前言	1
1.2研究動機	2
1.3幾丁質、幾丁聚醣簡介	3
1.3.1幾丁質、幾丁聚醣特性	3
1.3.2幾丁質與幾丁聚醣的製備	4
1.3.3幾丁聚醣的去乙醯化程度之測定	6
      1.3.4 測定分子量 .........................................................7
   1.4降解幾丁聚醣酵素之介紹..............................................8
   1.5 毛細管電泳簡介............................................................9
1.5.1毛細管電泳簡介	9
1.5.2毛細管電泳的分離原理	9
1.5.3毛細管電泳的分離方法	10
   1.6基質輔助雷射脫附游離質譜法(MALDI-TOF/MS)
簡介 ...............................................................................12
1.6本章參考資料	14
第二章 實驗	18
2.1實驗藥品	18
2.2實驗條件及步驟	21 
2.2.1毛細管電泳儀	21
2.2.2毛細管處理	22
2.2.3緩衝溶液配置	23
2.2.4以毛細管電泳測量之條件步驟	..24
2.2.5利用核磁共振法測量幾丁聚醣之去乙醯化程度	33
        2.2.6利用MALDI-TOF MS測量幾丁寡醣………..34
2.3實驗樣品備製	37 
2.3.1幾丁聚醣之去乙醯化反應	37
2.3.2幾丁聚醣之乙醯化反應	37
2.3.3幾丁聚醣之降解反應	39
2.4本章參考文獻	42
第三章 結果與討論	43
3.1幾丁聚醣去乙醯化程度	43
3.1.1緩衝溶液濃度對去乙醯化校正曲線的影響…..43
      3.1.2幾丁聚醣去乙醯化反應動力學 ………………52
3.1.3幾丁質去乙醯化反應動力學………………… .55
      3.1.4 不同去乙醯化幾丁聚醣混合物的測量 ...........59
      3.1.5測量實際幾丁聚醣藥品......................................61
      3.1.6 幾丁聚醣去乙醯化再乙醯化反應.....................64
  3.2 幾丁聚醣之分子量校正曲線........................................72
      3.2.1 建立幾丁聚醣分子量校正曲線.........................72
      3.2.2 不同濃度polymer buffer對校正曲線影響.......78
  3.3 幾丁聚醣降解反應....................................................... 82
      3.3.1 KPS降解條件..................................................... 82
      3.3.2 幾丁聚醣KPS降解反應動力學.........................88
3.3.3 酵素降解幾丁聚醣條件探討 ...........................92
3.4  MALDI-ToF-MS測量幾丁聚寡糖..............................96
3.5  結論............................................................................106
3.6 參考文獻......................................……………………108

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