酒精不管做為能源還是食物一直以來是人類依賴的重要產物，如何產酒以及增加產酒效率一直以來是工業中的課題之一。
    在過往的研究中，學家利用Methylnitronitrosoguanidine(MNNG)或Ethyl Methane Sulfonate(EMS)藥劑與UV突變法對酵母菌BY4742菌株進行基因突變，更有利用Global transcription machinery engineering （gTME）基因工程的方式轉換細胞基因，期望利用改變原有基因配置能增加酵母菌在高醣高酒精的環境下酒精生產的效率。
    本實驗採UV突變法並以3000、4000、5000μJ/cm2強度進行照射突變，再藉由高濃度葡萄糖與高濃度酒精的培養條件，對已隨機突變過的菌株進行連續液態篩選，初步篩選出具有高醣高酒精耐受性的菌種。

Alcohol has always been an important product of human dependence,
whether it is energy or food. How to produce wine and increase the efficiency of 
wine production has always been one of the topics in the industry.
    In previous studies, scientists used Methylnitronitrosoguanidine (MNNG) 
or Ethyl Methane Sulfonate (EMS) agents and UV mutations to genetically 
mutate the yeast BY4742 strain, furthermore, converted the genes of the cells by 
using Global transcription machinery engineering (gTME), expected to use the 
altered original gene configuration that can increase the efficiency of yeast 
production in high concentration of glucose and alcohol environments.
    In our experiment, UV mutation method was used to irradiate mutations 
with intensity of 3000, 4000 and 5000 μJ/cm2, and the strains with random 
mutations were subjected to continuous liquid screening by high-concentration 
glucose and high-concentration alcohol culture conditions. In order to find a 
strain with high sugar and high alcohol tolerance.

謝誌	I
中文摘要	II
英文摘要	III
總目錄	IV
圖表目錄	VI
第一章	緒論	1
1.1	前言	1
1.2	研究背景	1
1.3	研究動機	3
第二章	材料與方法	5
2.1	實驗材料	5
2.1.1	菌株	5
2.1.2	實驗藥品與溶液製備	5
2.1.2.1	藥品	5
2.1.2.2	溶液製備	6
2.1.3	實驗儀器	10
2.2	實驗方法	13
2.2.1	菌株突變	13
2.2.2	菌株篩選	14
2.2.3	生長能力測試	15
2.2.4	發酵能力測試	15
第三章	結果與討論	17
3.1	菌株突變	17
3.2	篩選方式	19
第四章	結論	22
第五章	參考資料	23
 
圖表目錄
圖1糖酵解與發酵過程	2
圖2酵母菌作為酒精發酵菌種的優勢與劣勢	4
圖3分光光度計	10
圖4 UV crosslinker	10
圖5倒立顯微鏡	11
圖6高壓滅菌釜	12
圖7酸鹼測量儀	12
圖8實驗流程	13
圖9滾珠圖盤分布情形	18
圖10使用UV突變致死率	19
圖11不同篩選條件下細胞密度	20

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