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系統識別號 U0002-2507201914570400
中文論文名稱 金銀花與蝶豆花水萃取液對人類纖維母細胞Hs68生長之效果
英文論文名稱 The effect of the water extract of Lonicera japonica and Clitoria ternatea on the growth efficiency of human fibroblast cell line Hs68
校院名稱 淡江大學
系所名稱(中) 化學學系碩士班
系所名稱(英) Department of Chemistry
學年度 107
學期 2
出版年 108
研究生中文姓名 林湘棋
研究生英文姓名 Shiang-Chi Lin
學號 606180072
學位類別 碩士
語文別 中文
第二語文別 英文
口試日期 2019-07-18
論文頁數 29頁
口試委員 指導教授-陳銘凱
委員-莊子超
委員-官宜靜
中文關鍵字 金銀花  蝶豆花  Hs68細胞  抗氧化  自由基 
英文關鍵字 Lonicera japonica  Clitoria ternatea  Hs68  antioxidant  free radicals 
學科別分類 學科別自然科學化學
中文摘要   金銀花(Lonicera japonica Thunb)是屬於忍冬科(Caprifoliaceae) 忍冬屬 (Lonicera)的草本植物,研究表明,金銀花具有抗氧化作用。
  蝶豆花(Clitoria ternatea )俗稱蝴蝶豌豆(Butterfly pea),是屬於豆科(Fabaceae)植物物種,亦有抗氧化作用。
  本論文旨在研究金銀花與蝶豆花水萃取物對Hs68細胞的生長效果,同時研究經過紫外線照射後產生自由基是否有抗氧化的能力。
英文摘要 Lonicera japonica Thunb , is an herbaceous plant of Caprifoliaceae family. Previous studies have demonstrated that Lonicera japonica has antioxidative activities.
Clitoria ternatea, commonly known as butterfly pea, is a plant species belonging to the family Fabaceae and also has antioxidant effects.
The purpose of this thesis is to study the growth effect of Lonicera japonica Thunb and Clitoria ternatea water extract on Hs68 cells, and to research whether the free radicals produced by ultraviolet light have anti-oxidation ability.
論文目次 第一章 緒論 1
1-1研究動機 1
1-2金銀花 (Lonicera japonica Thunb.) 2
1-3蝶豆花 ( Clitoria ternatea ) 3
1-4紫外線 (Ultraviolet, UV) 3
1-5自由基與活性氧化物 ( Free radical & Reactive oxygen species, ROS ) 4
1-6抗氧化物質 4
第二章 材料與方法 6
2-1實驗材料 6
2-1-1藥品與試劑 6
2-1-2實驗儀器 7
2-1-3細胞株 7
2-1-4樣品 7
2-2實驗方法 8
2-2-1萃取物製備 8
2-2-2人類上皮層纖維母細胞 ( Hs68 ) 之培養 8
2-2-3水萃物濃度篩選 9
2-2-4 UVC照射人類上皮層纖維母細胞 9
2-2-5細胞存活率分析 ( Cell Counting Kit 8 ) 10
2-2-6細胞內活性氧物質測定( Reactive oxygen species , ROS ) 10
2-2-7細胞免疫螢光染色 12
2-2-8自由基清除能力之測定 ( DPPH Assay ) 12
2-3 統計分析 12

第三章 實驗結果 13
PART1 金銀花水萃取物 13
3-1 確立人類上皮層纖維母細胞 (Hs68) 之紫外線暴露劑量 13
3-2 金銀花水萃取物對人類上皮層纖維母之細胞毒性 14
3-3金銀花水萃取物能減少 UVC 引起之細胞死亡 17
3-4細胞內ROS測定 18
3-5 DPPH自由基清除能力之測定 19
3-6螢光染色觀察經UVC處理後Hs68細胞產生變化 20
PART2 蝶豆花水萃取物 22
3-6 蝶豆花水萃取物對人類上皮層纖維母細胞的毒性作用 22
3-7 蝶豆花水萃取物是否一樣具有修復UVC能力 23
第四章 結論 24
第五章 參考文獻 26


圖目錄
圖1 UVC不同強度對Hs68細胞的毒殺作用 13
圖2 金銀花水萃取物對Hs68細胞之影響 14
圖3 金銀花水萃取物最佳濃度 15
圖4 金銀花水萃取物隨時間不同有抑制Hs68生長能力 16
圖5 金銀花水萃取物幫助UVC照射過後的細胞進行修復 17
圖6 細胞照射UVC後產生自由基 18
圖7 金銀花與蝶豆花水萃取物對DPPH 自由基清除能力之影響 19
圖8 Hs68細胞照射UV螢光染色圖 20
圖9 蝶豆花水萃取物之細胞存活率 22
圖10 蝶豆花水萃取物修復UVC傷害的功能 23
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