金銀花(Lonicera japonica Thunb)是屬於忍冬科(Caprifoliaceae) 忍冬屬 (Lonicera)的草本植物，研究表明，金銀花具有抗氧化作用。
　　蝶豆花(Clitoria ternatea )俗稱蝴蝶豌豆(Butterfly pea)，是屬於豆科(Fabaceae)植物物種，亦有抗氧化作用。
　　本論文旨在研究金銀花與蝶豆花水萃取物對Hs68細胞的生長效果，同時研究經過紫外線照射後產生自由基是否有抗氧化的能力。

Lonicera japonica Thunb , is an herbaceous plant of Caprifoliaceae family. Previous studies have demonstrated that Lonicera japonica has antioxidative activities.
Clitoria ternatea, commonly known as butterfly pea, is a plant species belonging to the family Fabaceae and also has antioxidant effects. 
The purpose of this thesis is to study the growth effect of Lonicera japonica Thunb and Clitoria ternatea water extract on Hs68 cells, and to research whether the free radicals produced by ultraviolet light have anti-oxidation ability.

第一章　緒論	1
1-1研究動機	1
1-2金銀花　(Lonicera japonica Thunb.)	2
1-3蝶豆花 ( Clitoria ternatea )	3
1-4紫外線 (Ultraviolet, UV)	3
1-5自由基與活性氧化物 ( Free radical & Reactive oxygen species, ROS )	4
1-6抗氧化物質	4
第二章 材料與方法	6
2-1實驗材料	6
2-1-1藥品與試劑	6
2-1-2實驗儀器	7
2-1-3細胞株	7
2-1-4樣品	7
2-2實驗方法	8
2-2-1萃取物製備	8
2-2-2人類上皮層纖維母細胞 ( Hs68 ) 之培養	8
2-2-3水萃物濃度篩選	9
2-2-4 UVC照射人類上皮層纖維母細胞	9
2-2-5細胞存活率分析 ( Cell Counting Kit 8 )	10
2-2-6細胞內活性氧物質測定( Reactive oxygen species , ROS )	10
2-2-7細胞免疫螢光染色	12
2-2-8自由基清除能力之測定 ( DPPH Assay )	12
2-3 統計分析	12

第三章 實驗結果	13
PART1 金銀花水萃取物	13
3-1 確立人類上皮層纖維母細胞 (Hs68) 之紫外線暴露劑量	13
3-2 金銀花水萃取物對人類上皮層纖維母之細胞毒性	14
3-3金銀花水萃取物能減少 UVC 引起之細胞死亡	17
3-4細胞內ROS測定	18
3-5 DPPH自由基清除能力之測定	19
3-6螢光染色觀察經UVC處理後Hs68細胞產生變化	20
PART2 蝶豆花水萃取物	22
3-6 蝶豆花水萃取物對人類上皮層纖維母細胞的毒性作用	22
3-7 蝶豆花水萃取物是否一樣具有修復UVC能力	23
第四章 結論	24
第五章 參考文獻	26

 
圖目錄
圖1 UVC不同強度對Hs68細胞的毒殺作用	13
圖2 金銀花水萃取物對Hs68細胞之影響	14
圖3 金銀花水萃取物最佳濃度	15
圖4 金銀花水萃取物隨時間不同有抑制Hs68生長能力	16
圖5 金銀花水萃取物幫助UVC照射過後的細胞進行修復	17
圖6 細胞照射UVC後產生自由基	18
圖7 金銀花與蝶豆花水萃取物對DPPH 自由基清除能力之影響	19
圖8 Hs68細胞照射UV螢光染色圖	20
圖9 蝶豆花水萃取物之細胞存活率	22
圖10 蝶豆花水萃取物修復UVC傷害的功能	23

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