系統識別號 | U0002-2507201315313800 |
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DOI | 10.6846/TKU.2013.01053 |
論文名稱(中文) | 一新穎5,7-Dihydroxy-3’,4’-Diaminoflavone抑制HER2表現並抑制HER2過表現人類卵巢癌細胞的轉移能力 |
論文名稱(英文) | A novel 5,7-Dihydroxy-3’,4’-Diaminoflavone downregulates HER2 expression and represses metastasis-associated properties in HER2-overexpressing ovarian cancer cells |
第三語言論文名稱 | |
校院名稱 | 淡江大學 |
系所名稱(中文) | 化學學系碩士班 |
系所名稱(英文) | Department of Chemistry |
外國學位學校名稱 | |
外國學位學院名稱 | |
外國學位研究所名稱 | |
學年度 | 101 |
學期 | 2 |
出版年 | 102 |
研究生(中文) | 鄭欣怡 |
研究生(英文) | Hsin-Yi Cheng |
學號 | 600180177 |
學位類別 | 碩士 |
語言別 | 繁體中文 |
第二語言別 | |
口試日期 | 2013-06-21 |
論文頁數 | 45頁 |
口試委員 |
指導教授
-
莊子超
委員 - 高銘欽 委員 - 施增廉 委員 - 莊子超 |
關鍵字(中) |
人類表皮生長因子受體2 |
關鍵字(英) |
HER2 |
第三語言關鍵字 | |
學科別分類 | |
中文摘要 |
類黃酮已經被研究出許多抗癌活性,包括抗增生、促進細胞凋亡、抗血管新生和細胞週期阻斷。約有30%的乳癌和卵巢癌病人有HER2基因放大或是HER2過表現的現象,且HER2過表現和細胞轉移及抗藥性有關。一系列新穎的黃酮類化合物被用來探討其對癌細胞的抗癌作用。我們發現了Cpd3 (5,7-Dihydroxy-3’,4’-Diaminoflavone)在HER2過表現癌細胞中有明顯的抗癌活性,包括抗增生、抗轉移以及促進細胞凋亡。此外,Cpd3可以增加HER2過表現細胞細胞中的HER2降解。這些結果表明,Cpd3可能是透過降低HER2的表現量以抑制HER2過表現細胞的細胞生長。 |
英文摘要 |
Flavonoids have been studied for their anti-tumor properties, including anti-proliferative, proapoptotic, anti-angiogenic and cell cycle arrest activities in several cancer cells. Her2 is amplified and/or overexpressed in about 30% of ovarian and breast cancers and is associated with metastasis and drug resistance. A series of novel flavones were used to explore their anticancer effects on cancer cells. We found a 5,7-Dihydroxy-3’,4’-Diaminoflavone, Cpd3, with significant anti-tumor activities in HER2-overexpressing cancer cells, including anti-proliferative, anti-metastatic, and proapoptotic activities. Moreover, Cpd3 could increase the HER2 degradation in HER2-overexpressing cells. These data suggest that cpd3 suppress the HER2-overexpressing cancer cells growth may via down-regulation of HER2. |
第三語言摘要 | |
論文目次 |
謝誌 I 縮寫表 IV 目錄 V 第一章 緒論 1 第一節HER2/neu 1 1-1 HER2/neu的簡介 1 1-2 HER2和癌症間的關係 3 1-3 HER2的訊號路徑 3 1-4 以HER2為目標的治療方式 4 1-4-1單株抗體 (monoclonal antibodies, mAbs) 5 1-4-2 酪胺酸激酶抑制劑 (tyrosine kinase inhibitors) 6 1-4-3 Hsp90 (Heat shock protein 90) 抑制劑 6 第二節 黃酮類化合物 7 2-1黃酮類的簡介 7 2-2黃酮類和癌症的關係 9 研究動機 11 第二章 材料與方法 12 第一節 材料 12 1-1 試劑 (Reagent) 12 1-2 抗體 (Antibody) 13 第二節 方法 13 第三章 結果 21 第一節實驗結果 21 第二節 實驗圖表 25 第四章 討論 32 第五章 參考資料 34 附錄一 42 附錄二 45 |
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