類黃酮已經被研究出許多抗癌活性，包括抗增生、促進細胞凋亡、抗血管新生和細胞週期阻斷。約有30%的乳癌和卵巢癌病人有HER2基因放大或是HER2過表現的現象，且HER2過表現和細胞轉移及抗藥性有關。一系列新穎的黃酮類化合物被用來探討其對癌細胞的抗癌作用。我們發現了Cpd3 (5,7-Dihydroxy-3’,4’-Diaminoflavone)在HER2過表現癌細胞中有明顯的抗癌活性，包括抗增生、抗轉移以及促進細胞凋亡。此外，Cpd3可以增加HER2過表現細胞細胞中的HER2降解。這些結果表明，Cpd3可能是透過降低HER2的表現量以抑制HER2過表現細胞的細胞生長。

Flavonoids have been studied for their anti-tumor properties, including anti-proliferative, proapoptotic, anti-angiogenic and cell cycle arrest activities in several cancer cells. Her2 is amplified and/or overexpressed in about 30% of ovarian and breast cancers and is associated with metastasis and drug resistance. A series of novel flavones were used to explore their anticancer effects on cancer cells. We found a 5,7-Dihydroxy-3’,4’-Diaminoflavone, Cpd3, with significant anti-tumor activities in HER2-overexpressing cancer cells, including anti-proliferative, anti-metastatic, and proapoptotic activities. Moreover, Cpd3 could increase the HER2 degradation in HER2-overexpressing cells. These data suggest that cpd3 suppress the HER2-overexpressing cancer cells growth may via down-regulation of HER2.

謝誌	I
縮寫表	IV
目錄	V
第一章	緒論	1
第一節HER2/neu	1
1-1 HER2/neu的簡介	1
1-2 HER2和癌症間的關係	3
1-3 HER2的訊號路徑	3
1-4 以HER2為目標的治療方式	4
1-4-1單株抗體 (monoclonal antibodies, mAbs)	5
1-4-2 酪胺酸激酶抑制劑 (tyrosine kinase inhibitors)	6
1-4-3 Hsp90 (Heat shock protein 90) 抑制劑	6
第二節 黃酮類化合物	7
2-1黃酮類的簡介	7
2-2黃酮類和癌症的關係	9
研究動機	11
第二章	材料與方法	12
第一節 材料	12
1-1 試劑 (Reagent)	12
1-2 抗體 (Antibody)	13
第二節 方法	13
第三章	結果	21
第一節實驗結果	21
第二節 實驗圖表	25
第四章	討論	32
第五章 參考資料	34
附錄一	42
附錄二	45

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方冠勳碩士論文，2009，淡江大學，台北，中華民國。