淡江大學覺生紀念圖書館 (TKU Library)

系統識別號 U0002-2507201315313800
中文論文名稱 一新穎5,7-Dihydroxy-3’,4’-Diaminoflavone抑制HER2表現並抑制HER2過表現人類卵巢癌細胞的轉移能力
英文論文名稱 A novel 5,7-Dihydroxy-3’,4’-Diaminoflavone downregulates HER2 expression and represses metastasis-associated properties in HER2-overexpressing ovarian cancer cells
校院名稱 淡江大學
系所名稱(中) 化學學系碩士班
系所名稱(英) Department of Chemistry
學年度 101
學期 2
出版年 102
研究生中文姓名 鄭欣怡
研究生英文姓名 Hsin-Yi Cheng
學號 600180177
學位類別 碩士
語文別 中文
口試日期 2013-06-21
論文頁數 45頁
口試委員 指導教授-莊子超
中文關鍵字 人類表皮生長因子受體2 
英文關鍵字 HER2 
學科別分類 學科別自然科學化學
中文摘要 類黃酮已經被研究出許多抗癌活性,包括抗增生、促進細胞凋亡、抗血管新生和細胞週期阻斷。約有30%的乳癌和卵巢癌病人有HER2基因放大或是HER2過表現的現象,且HER2過表現和細胞轉移及抗藥性有關。一系列新穎的黃酮類化合物被用來探討其對癌細胞的抗癌作用。我們發現了Cpd3 (5,7-Dihydroxy-3’,4’-Diaminoflavone)在HER2過表現癌細胞中有明顯的抗癌活性,包括抗增生、抗轉移以及促進細胞凋亡。此外,Cpd3可以增加HER2過表現細胞細胞中的HER2降解。這些結果表明,Cpd3可能是透過降低HER2的表現量以抑制HER2過表現細胞的細胞生長。
英文摘要 Flavonoids have been studied for their anti-tumor properties, including anti-proliferative, proapoptotic, anti-angiogenic and cell cycle arrest activities in several cancer cells. Her2 is amplified and/or overexpressed in about 30% of ovarian and breast cancers and is associated with metastasis and drug resistance. A series of novel flavones were used to explore their anticancer effects on cancer cells. We found a 5,7-Dihydroxy-3’,4’-Diaminoflavone, Cpd3, with significant anti-tumor activities in HER2-overexpressing cancer cells, including anti-proliferative, anti-metastatic, and proapoptotic activities. Moreover, Cpd3 could increase the HER2 degradation in HER2-overexpressing cells. These data suggest that cpd3 suppress the HER2-overexpressing cancer cells growth may via down-regulation of HER2.
論文目次 謝誌 I
縮寫表 IV
目錄 V
第一章 緒論 1
第一節HER2/neu 1
1-1 HER2/neu的簡介 1
1-2 HER2和癌症間的關係 3
1-3 HER2的訊號路徑 3
1-4 以HER2為目標的治療方式 4
1-4-1單株抗體 (monoclonal antibodies, mAbs) 5
1-4-2 酪胺酸激酶抑制劑 (tyrosine kinase inhibitors) 6
1-4-3 Hsp90 (Heat shock protein 90) 抑制劑 6
第二節 黃酮類化合物 7
2-1黃酮類的簡介 7
2-2黃酮類和癌症的關係 9
研究動機 11
第二章 材料與方法 12
第一節 材料 12
1-1 試劑 (Reagent) 12
1-2 抗體 (Antibody) 13
第二節 方法 13
第三章 結果 21
第一節實驗結果 21
第二節 實驗圖表 25
第四章 討論 32
第五章 參考資料 34
附錄一 42
附錄二 45
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