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系統識別號 U0002-2107201000135100
中文論文名稱 3,3'-二吲哚甲烷在卵巢癌細胞中之抗細胞增生作用
英文論文名稱 The antiproliferation effect of 3,3'-Diindolymethane in ovarian cancer cell lines.
校院名稱 淡江大學
系所名稱(中) 生命科學研究所碩士班
系所名稱(英) Graduate Institute of Life Sciences
學年度 98
學期 2
出版年 99
研究生中文姓名 陳怡雯
研究生英文姓名 Yi-Wen Chen
學號 696180396
學位類別 碩士
語文別 中文
口試日期 2010-06-23
論文頁數 44頁
口試委員 指導教授-莊子超
委員-陳銘凱
委員-許世忠
中文關鍵字 3,3'-二吲哚甲烷  抗增生  卵巢癌細胞 
英文關鍵字 DIM  antiproliferation  ovarian cancer cell 
學科別分類 學科別醫學與生命科學生物學
中文摘要 過去的研究指出3,3'-二吲哚甲烷具有抗癌的效果。本實驗分析3,3'-二吲哚甲烷在不同HER2及p53蛋白質含量的卵巢癌細胞之抗細胞增生效果。這個研究發現,3,3'-二吲哚甲烷可能透過延滯細胞週期造成抑制卵巢癌細胞的生長。3,3'-二吲哚甲烷透過改變Cyclin dependent kinases inhibitors (p21, and/or p27)的蛋白質含量,抑制了MDAH-2774、SKOV-3及TOV-21G的增生。總結,這研究發現3,3'-二吲哚甲烷可能具有發展成化學預防和治療卵巢癌劑的能力。
英文摘要 3,3'-Diindolylmethane (DIM) has been reported to exhibit anti-tumor activities. Here I had examined the anti-proliferating effects of 3,3'-diindolylmethane on different HER2 and p53 protein levels of ovarian cancer cell lines. This study found that 3,3'-diindolylmethane inhibit ovarian cancer cells growth may via cycle arrest. Inhibition of MDAH-2774, SKOV-3 and TOV-21G ovarian cells proliferation by DIM depends on changes in Cyclin dependent kinases inhibitors (p21, and/or p27). In summary, these findings suggest that DIM may be a potential chemopreventive and therapeutic agent for ovarian cancer.
論文目次 第一章 緒論……………………………………………………………1
第一節 卵巢癌細胞簡介………………………………………………1
第二節 3,3'-二吲哚甲烷簡介…………………………………………2
第三節 細胞週期的概論………………………………………………5
第四節 研究動機與目的 ……………………………………………15

第二章 材料與方法……………………………………………16
第一節 實驗材料………………………………………………………16
第二節 實驗方法………………………………………………………18

第三章 結果……………………………………………………21
第一節 實驗結果……………………………………………………21
第二節 實驗圖表……………………………………………………24

第四章 結論與討論……………………………………………33
第五章 參考文獻…………………………………………………36
附錄 43

圖目錄
圖一3,3'-二吲哚甲烷對卵巢癌細胞SKOV-3、TOV-21G、MDAH-2774 的外觀形態上之影響………………………………………………..…24
圖二3,3'-二吲哚甲烷對卵巢癌細胞SKOV-3、TOV-21G、MDAH-2774 之細胞生長影響……………………………………..…………………27
圖三 3,3'-二吲哚甲烷對卵巢癌細胞SKOV-3、TOV-21G、MDAH-2774之pAKT及AKT蛋白質的影響…………………………….……..….28
圖四 3,3'-二吲哚甲烷對卵巢癌細胞SKOV-3、TOV-21G、MDAH-2774的CDKI蛋白質影響…………………………………….……………..29
圖五 3,3'-二吲哚甲烷對卵巢癌細胞SKOV-3、TOV-21G、MDAH-2774的G1期之Cyclin及CDK蛋白質影響…….………………………….30
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3,3'-二吲哚甲烷抑制神經膠原致癌基因表現和轉移特性之研究,施伊珊,2008淡江大學碩士論文
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