使用幾丁類物質粗製蝦殼粉 (Crude SSC) 與磁性幾丁聚醣/四氧化三鐵納米複合顆粒 (NF) 以吸附方式純化酵素，以達成快速純化之目的。
    由吸附劑SP、AC、pure chitin、Crude SSC、Crude CSC、Crude SPC中選擇較適吸附劑Crude SSC。再探討溫度、重量、時間對吸附鳳梨酵素之影響。經由實驗得知於4℃、30分鐘、0.1 g的條件下，得到比活性0.0918 U/mg。
    再探討溫度、重量、時間對NF吸附鳳梨酵素之影響。從實驗得知於25℃、30分鐘、0.1 g 的條件，得到比活性0.1357 U/mg。
    使用上述條件，使用NF與Crude SSC吸附細菌酵素，藉此討論吸附方式純化酵素的結果。經由實驗可知純化酵素影響條件並非只有等電點的因素，也需考慮酵素和吸附劑的相互作用還有物理吸附等因素。
    結果證明吸附方式純化酵素是一個可行的純化方式，可以提高純化效率。

Application of NF and Crude SSC on the purification of enzymes to achieve rapid purification purposes. Adsorbent : SP, AC, pure chitin, Crude SSC, Crude CSC, Crude SPC , Choose a more suitable adsorbent Crude SSC. Studying temperature, weight, time on the adsorption effect of the pineapple enzyme. By experiment that at 4 ℃, 30 min, 0.1 g of the conditions to get specific activity 0.0918 U / mg.
  Studying temperature, weight, time on the effect of NF adsorption bromelain enzyme. Learned from the experiment at 25 ℃, 30 min, 0.1 g of the conditions to get specific activity 0.1357 U / mg.
  Use the above conditions, NF, and Crude SSC absorption bacterial enzyme purified enzyme to discuss the results. Purified enzyme affected by the experimental conditions known isoelectric point is not the only factor, but also need to consider the interaction between enzyme and adsorbents as well as physical absorption and other factors.
  Results show that absorption is a viable way to purify the enzymes, it can improve the purification efficiency.

第一章 緒論	1
第二章 文獻回顧	2
2.2 幾丁聚醣之應用	2
2.3蛋白酶	3
2.4幾丁質酶及幾丁聚醣酶	3
2.5吸附劑	4
2.6 吸附劑種類	5
2.7 幾丁質吸附劑	5
2.8磁性幾丁聚醣/四氧化三鐵奈米複合顆粒 (NP) 特性	6
2.9 吸附及純化	6
第三章 材料與方法	8
3.1 實驗材料	8
3.2 實驗儀器	9
3.3 吸附流程	9
3.4 鳳梨酵素的製備	12
3.5 蛋白酶之活性測定	12
3.6 幾丁聚醣酶之活性測定	12
3.7 crude SSC之較適吸附條件之探討	13
3.7.1吸附劑選擇	13
3.7.2 crude SSC吸附濃度	13
3.5.3 crude SSC吸附溫度	14
3.8 NP較適吸附條件之探討	14
3.8.1 顆粒大小之選擇	14
3.8.2 NP添加量之選擇	14
3.8.3 NP吸附溫度	14
3.9 crude SSC和NP於細菌酵素之吸附	15
3.9.1粗酵素液製備	15
3.9.2 粗酵素液之吸附	15
3.9.3 電泳分析	15
第四章 結果與討論	16
4.1吸附劑及吸附條件探討	16
4.1.1 吸附劑之探討	16
4.1.2吸附劑濃度對於吸附鳳梨酵素之影響	17
4.1.3溫度對於吸附之影響	18
4.2 NP之吸附條件探討	20
4.2.1 顆粒大小對於吸附之影響	20
4.2.2 NP添加量於吸附之影響	20
4.2.3溫度對於NP吸附的影響	21
4.2.4時間對於NP吸附之影響	21
4.3 crude SSC與NP吸附細菌酵素之條件探討	23
4.3.1 TKU 011粗酵素液之吸附結果	23
4.3.2 TKU 015粗酵素液之吸附結果	24
4.3.3 TKU 022粗酵素液之吸附結果	24
4.3.4吸附TKU 024粗酵素液之結果	25
4.4細菌酵素吸附之比較	26
第五章 結論	44
參考資料	45

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