利用帶有四碳鏈的膠體帶有芳香烴的膠體分離重組蛋白。以不同的進樣方向，配合不同鹽的濃度和停留在膠體的時間，找出並測試對樣品分離的前後順序條件。

The use of colloidal gel with four-carbon chain and colloidal gel with aromatic hydrocarbon to separate recombinant proteins were studied. In a different direction of injection, with different salt concentration and residence time in the colloidal gel, the orders of separation of samples were tested and identified.

謝誌 I
中文摘要 II
英文摘要 III
縮寫檢表 IV

第一章  緒論	1
蛋白質體學	1
研究目的	2
第二章:材料和方法	3
2.1 實驗器材與藥品	3
2.1.1 實驗藥品	3
2.1.2 實驗儀器 	4
2.2 誘導蛋白質表現	5
2.2.1 誘導 NNT1p 的表現	5
2.2.2 誘導 CYS4p 的表現	5
2.2.3 誘導 SEE1p表現	6
2.2.4 誘導 UBC4p 的表現	6
2.2.4 誘導 YNR029Cp 的表現	7
2.3 配置實驗所需Buffer	8
2.4 超音波破菌法	8
管柱裝填	9
2.5 蛋白質倒沖純化:	10
2.6 蛋白質正沖純化:	10
管柱清洗:	11
2.7 SDS蛋白質電泳:	11
第三章:實驗之結果分析	12
CYS4p	12
SEE1p	12
NNT1p	13
UBC4p	13
第四章:討論	14
正沖與倒沖之比較	14
疏水性比較	15
第五章:結論	18
第六章:參考文獻	19
第七章:圖	21
圖.1 YNR029Cp在不同(NH4)2SO4濃度下破菌的表現量。	21
圖.2 a CYS4p在Phenyl Sepharose 6 Fast Flow，倒沖與正沖wash。	22
圖.2 b CYS4p在Phenyl Sepharose 6 Fast Flow，倒沖與正沖elute。	23
圖.3 a SEE1p 在Phenyl Sepharose 6 Fast Flow，倒沖與正沖wash。	24
圖.3 b SEE1p 在Phenyl Sepharose 6 Fast Flow，倒沖與正沖elute。	25
圖.4 a NNT1p 在Butyl-S Sepharose 6 Fast Flow，倒沖與正沖wash。	26
圖.4 b NNT1p 在Butyl-S Sepharose 6 Fast Flow，倒沖與正沖elute。	27
圖.5 a UBC4p 在Butyl-S Sepharose 6 Fast Flow，倒沖與正沖wash。	28
圖.5 b UBC4p 在Butyl-S Sepharose 6 Fast Flow，倒沖與正沖elute。	29

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