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系統識別號 U0002-1807201210240500
中文論文名稱 黃芩素誘導人類乳癌細胞之細胞分化
英文論文名稱 Baicalein induces Differentiation in Human Breast Cancer Cells
校院名稱 淡江大學
系所名稱(中) 化學學系碩士班
系所名稱(英) Department of Chemistry
學年度 100
學期 2
出版年 101
研究生中文姓名 林芳琳
研究生英文姓名 Fang-Lin Lin
學號 699180013
學位類別 碩士
語文別 中文
口試日期 2012-06-28
論文頁數 52頁
口試委員 指導教授-莊子超
委員-高銘欽
委員-金亭佑
中文關鍵字 黃芩素  細胞分化 
英文關鍵字 Baicalein  Differentiation 
學科別分類 學科別自然科學化學
中文摘要 由傳統中藥材黃芩根部所萃取的類黃酮化合物-黃芩素(Baicalein),在許多文獻中已證實具抗氧化、抗發炎與抗癌的活性。在本研究中,我們探討黃芩素對於乳癌細胞的化學預防效果。使用紅油O(Oil Red O)及尼羅紅(Nile red) 螢光染色,觀察到黃芩素誘發乳癌細胞內油滴以及中性脂肪的產生隨著時間與濃度的增加而增加。利用西方墨點法偵測乳房上皮細胞分化指標蛋白的變化,發現黃芩素可誘發乳癌細胞內Milk fat globulin和Cytokeratin 8 等分化指標分子表現量增加。進一步發現黃芩素所誘發乳癌細胞分化的現象受HER2過表現而降低。綜和實驗結果證實黃芩素作用於乳癌細胞株,具引發乳癌細胞分化的活性。
英文摘要 Baicalein, a flavonoid contained in the herbal medicine, extracted from root of Scutellaria baicalensis was found to exert antioxidant, anti-inflammation and antitumor activities. Here, we explored the chemopreventive activity of baicalein in breast cancer cells. By using Oil Red O and Nile red staining, we found that baicalein caused neutral lipids accumulation in dose- and time-dependent manners. Besides, induction of milk fat globulin and cytokeratin 8, makers of mature breast cells, after treating with baicalein were also observed. Furthermore, introduction of HER2 into these breast cells reversed the baicalein induced differentiation. Taken together, our data indicate that baicalein suppresses growth and induces differentiation of breast cancer cells.
論文目次 目錄
第一章 緒論................................................1
第一節 細胞分化...........................................1
第二節 黃芩素.............................................7
第二章 實驗目的.............................................16
第三章 材料與方法............................................17
第四章 結果...............................................21
第一節 實驗結果.........................................21
第二節 實驗圖表..........................................24
第五章 討論................................................33
第六章 參考文獻.............................................40
附錄一......................................................51
附錄二......................................................52
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方冠勛, 2009淡江大學碩士論文,黃芩素在神經膠原致癌基因過表現卵巢癌細胞中的抗癌效果
邵韋勳, 2011淡江大學碩士論文,人類卵巢癌細胞中Chk2蛋白的活化有助於黃芩素所誘導之G2/M細胞週期停滯
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