致癌基因 HER2/neu 過表現導致腫瘤細胞侵入、轉移和血管新生。文獻指出， 3,3’-二吲哚甲烷 (3,3’-diindolylmethane, DIM) 擁有抗癌活性。本實驗證實 DIM 能夠抑制 HER2/neu 表現的卵巢癌和乳癌細胞增生。本實驗結果顯示 DIM 在 HER2/neu 表現的卵巢癌和乳癌細胞中，能抑制 HER2/neu 和其下游 PI3K/Akt 訊號傳遞路徑，因此降低 HER2/neu 誘導的腫瘤轉移能力。此外從 PARP 被切斷的程度顯示 DIM 能促進卵巢癌和乳癌細胞凋亡。總和以上結果，本實驗為 DIM 的抗癌效應提出新的分子機制。

Overexpression of the HER2 oncogene contributes to tumor cell invasion, metastasis and angiogenesis. 3,3’-diindolylmethane (DIM) has been reported to exhibit anti-tumor activities. Here, we examined the anti-proliferating effects of DIM on HER2-positive ovarian and breast cancer cells. Our results demonstrated that DIM may inhibit HER2 and its downstream PI3K/Akt-signaling network, thus resulting in suppression of HER2-mediated metastatic potential in HER2-positive ovarian and breast cancers. Besides, DIM-induced apoptosis in HER2-positive ovarian and breast cancer cells was characterized by the up-regulation of cleaved poly(ADP-ribose) polymerase (PARP). Taken together, our data provide a new mechanism for the DIM-mediated anticancer effect.

摘要	I
中文摘要	I
英文摘要	II
第一章 緒論	1
第一節 神經膠原致癌基因 (HER2/NEU)	1
第二節 3, 3’-二吲哚甲烷	11
第三節 實驗動機與目的	19
第二章 材料與方法	20
第三章 結果	29
第一節 實驗結果	29
第二節 實驗圖表	35
第四章 討論	46
第五章 參考文獻	49
附錄	68

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