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中文論文名稱 3,3'-二吲哚甲烷藉由降低 HER2/neu 誘使 HER2/neu 過表現卵巢癌和乳癌細胞凋亡
英文論文名稱 3, 3’-diindolylmethane induces apoptosis through reduction of HER2/neu in HER2/neu-overexpressing ovarian and breast cancers
校院名稱 淡江大學
系所名稱(中) 化學學系碩士班
系所名稱(英) Department of Chemistry
學年度 99
學期 2
出版年 100
研究生中文姓名 梁展帆
研究生英文姓名 Chan-Fan Liang
學號 697160892
學位類別 碩士
語文別 中文
口試日期 2011-05-19
論文頁數 73頁
口試委員 指導教授-莊子超
委員-許世忠
委員-陳銘凱
中文關鍵字 神經膠原致癌基因  3, 3'-二吲哚甲烷 
英文關鍵字 HER2/neu  3,3'-diindolylmethe 
學科別分類 學科別自然科學化學
中文摘要 致癌基因 HER2/neu 過表現導致腫瘤細胞侵入、轉移和血管新生。文獻指出, 3,3’-二吲哚甲烷 (3,3’-diindolylmethane, DIM) 擁有抗癌活性。本實驗證實 DIM 能夠抑制 HER2/neu 表現的卵巢癌和乳癌細胞增生。本實驗結果顯示 DIM 在 HER2/neu 表現的卵巢癌和乳癌細胞中,能抑制 HER2/neu 和其下游 PI3K/Akt 訊號傳遞路徑,因此降低 HER2/neu 誘導的腫瘤轉移能力。此外從 PARP 被切斷的程度顯示 DIM 能促進卵巢癌和乳癌細胞凋亡。總和以上結果,本實驗為 DIM 的抗癌效應提出新的分子機制。
英文摘要 Overexpression of the HER2 oncogene contributes to tumor cell invasion, metastasis and angiogenesis. 3,3’-diindolylmethane (DIM) has been reported to exhibit anti-tumor activities. Here, we examined the anti-proliferating effects of DIM on HER2-positive ovarian and breast cancer cells. Our results demonstrated that DIM may inhibit HER2 and its downstream PI3K/Akt-signaling network, thus resulting in suppression of HER2-mediated metastatic potential in HER2-positive ovarian and breast cancers. Besides, DIM-induced apoptosis in HER2-positive ovarian and breast cancer cells was characterized by the up-regulation of cleaved poly(ADP-ribose) polymerase (PARP). Taken together, our data provide a new mechanism for the DIM-mediated anticancer effect.
論文目次 摘要 I
中文摘要 I
英文摘要 II
第一章 緒論 1
第一節 神經膠原致癌基因 (HER2/NEU) 1
第二節 3, 3’-二吲哚甲烷 11
第三節 實驗動機與目的 19
第二章 材料與方法 20
第三章 結果 29
第一節 實驗結果 29
第二節 實驗圖表 35
第四章 討論 46
第五章 參考文獻 49
附錄 68
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