本實驗採用一透析過濾裝置進行實驗，以單成分HA溶液、單成分LY溶液以及HA/LY雙成分溶液為實驗對象，並採用批次恆壓型式，針對攪拌速度、電解質種類、電解質濃度、透析液體積、透析液電解質濃度等操作變數之改變進行實驗分析。
研究結果顯示，加入攪拌可有效降低過濾阻力而提高濾速，電解質離子強度越大，使玻尿酸分子萎縮捲曲的程度越大，使得HA堆積形態越為緊密，濾速相對較低；而電解質造成LY與薄膜以及HA之間的靜電作用力降低，使LY不易吸附於膜面上，較易通過薄膜，提高移除效率。隨著透析過濾操作之進行，增加透析體積有助於批次濾出進料槽中之LY；若透析液中電解質濃增加有助於LY之移除率，但到達一定濃度後，再增加電解質濃度反而會影響LY的移除。

In this study, the HA solution, LY solution and the mixture solution of HA/LY were used as the feed solution, and experimented by adopting diafiltration in a dead-end stirred cell. The experimented results were discussed under different operating conditions such as stirred rate, electrolyte type, electrolyte concentration and diavolumes.
The results shows that the addition of stirring can reduce the filtration resistance  and improve the permeate flux. The permeate flux decreases with the increase of ion strength of electrolyte which causes the HA molecules become more shrunken and then form a compact deposited layer on membrane surface. However, the LY molecules can pass through the membrane more easily as the electrolyte is added. The strength of electrode ion will hinder the surface charge of LY, and decrease the electrostatic interference between LY molecules and membrane. During the diafiltration process of HA/LY solution, the diavolumes and electrolyte concentration can increase the removal of LY. But there is a limit to the increase of electrolyte concentration in dialyate solution, instead, would decrease the removal of LY.

目錄
致謝	I
中文摘要	II
英文摘要	III
目錄	IV
圖目錄	VII
表目錄	X
第1章 緒論	1
1.1 前言	1
1.2 薄膜分離	1
1.3 薄膜型態與模組	4
1.4 濃度極化與結垢現象	6
1.5 研究目的	7
第2章 文獻回顧	11
2.1 玻尿酸	11
2.1.1 玻尿酸之發現	12
2.1.2 玻尿酸之純化	13
2.1.3 玻尿酸之應用	15
2.2 透析過濾相關研究	17
2.2.1 透析過濾之理論分析	17
2.2.2 透析過濾純化效果之研究	18
2.2.3 透析過濾之應用	19
2.3 影響濾速之因素	20
2.4 提升濾速之方法	21
2.5 濾速分析模式	23
第3章 實驗裝置與方法	29
3.1 實驗裝置	29
3.2 實驗藥品	30
3.3 實驗步驟	31
3.4 操作條件	32
3.5 分析方法	33
3.5.1 玻尿酸含量之測定	33
3.5.2 蛋白質含量之測定	34
3.5.3 阻隔率(Rejection, Rj)	36
3.5.4 移除率(Reduction, Rd)	36
3.5.5 保留液濃度	36
3.6 實驗後薄膜之清洗	37
第4章 結果與討論	40
4.1 薄膜純水濾速	40
4.2 單成分溶液之透析過濾行為	41
4.2.1 攪拌速度對單成份溶液之影響	41
4.2.2 電解質對單成分溶液之影響	42
4.3 雙成分HA/LY溶液之透析過濾行為	43
4.3.1 電解質種類	43
4.3.2 電解質濃度	44
4.3.3 透析液體積	45
4.3.4 透析液電解質濃度	45
4.3.5 分離效率	46
4.3.6 電解質之移除	48
4.3.7 HA之阻隔率	49
4.3.8 移除雙成分溶液中微量蛋白質	49
第5章 結論	67
參考文獻	69
附錄A	76
附錄B	77

圖目錄
Fig. 1 1 The classification of membrane separation process [2].	9
Fig. 1 2 The diagram of (a)dead end filtration and (b)cross-flow filtration [3].	10
Fig. 2 1 The biosynthetic pathway for HA production in S. zooepidemicus[53].	25
Fig. 2 2 The processes of HA production in S. zooepidemicus[53].	26
Fig. 2 3 The Schematic diagram of diafiltration.	27
Fig. 2 4 Typical methods to reduce concentration polarization and fouling in pressure driving membrane processes [2].	28
Fig. 3 1 The experimental set-up of diafiltration operation.	38
Fig. 4 1 Pure water fluxes at different pressures of 100 kDa membrane.	50
Fig. 4 2 Time courses filtration flux on diafiltration of HA solution under different Stirring rates.	50
Fig. 4 3 Time courses filtration resistances on diafiltration of HA solution under different Stirring rates.	51
Fig. 4 4 Time courses filtration resistances on diafiltration of HA solution under 0 rpm.	51
Fig. 4 5 Time courses filtration flux on diafiltration of LY solution under different Stirring rates.	52
Fig. 4 6 Time courses filtration resistances on diafiltration of LY solution under different Stirring rates.	52
Fig. 4 7 Effect of different Stirring rates on LY conc. in permeate of LY solution.	53
Fig. 4 8 Time courses filtration flux on diafiltration of HA solution under different electrolyte concentrations.	53
Fig. 4 9 Time courses filtration resistances on diafiltration of HA solution under different electrolyte concentrations.	54
Fig. 4 10Time courses filtration flux on diafiltration of LY solution under different electrolyte concentrations.	54
Fig. 4 11Time courses filtration resistances on diafiltration of LY solution under different electrolyte concentrations.	55
Fig. 4 12 Effect of different electrolyte concentrations on LY conc. in permeate of LY solution.	55
Fig. 4 13 Time courses filtration flux on diafiltration of binary HA/LY solution under different electrolytes.	56
Fig. 4 14Time courses filtration resistances on diafiltration of binary HA/LY solution under different electrolytes.	56
Fig. 4 15 Time courses filtration flux on diafiltration of binary HA/LY solution under different electrolytes.	57
Fig. 4 16 Time courses filtration resistances on diafiltration of binary HA/LY solution under different electrolytes.	57
Fig. 4 17 Time courses filtration flux on diafiltration of binary HA/LY solution under different electrolytes.	58
Fig. 4 18 Time courses filtration resistances on diafiltration of binary HA/LY solution under different electrolytes.	58
Fig. 4 19 Time courses filtration flux on diafiltration of binary HA/LY solution under different electrolyte (KCl) concentrations.	59
Fig. 4 20 Time courses filtration resistances on diafiltration of binary HA/LY solution under different electrolyte (KCl) concentrations.	59
Fig. 4 21 Time courses filtration flux on diafiltration of binary HA/LY solution under different electrolyte (MgCl2) concentrations.	60
Fig. 4 22 Time courses filtration resistances on diafiltration of binary HA/LY solution under different electrolyte (MgCl2) concentrations.	60
Fig. 4 23 Time courses filtration flux on diafiltration of binary HA/LY solution under different diavolumes.	61
Fig. 4 24 Time courses filtration resistances on diafiltration of binary HA/LY solution under different diavolumes.	61
Fig. 4 25 Time courses filtration flux on diafiltration of binary HA/LY solution under different electrolyte concentrations of dialysate.	62
Fig. 4 26 Time courses filtration resistances on diafiltration of binary HA/LY solution under different electrolyte concentrations of dialysate.	62
Fig. 4 27 Effect of different electrolytes on LY conc. in permeate of binary HA/LY solution.	63
Fig. 4 28 Effect of different electrolyte concentrations on LY conc. in permeate of binary HA/LY solution.	63
Fig. 4 29 Effect of different diavolumes on LY conc. in permeate of binary HA/LY solution.	64
Fig. 4 30 Effect of different electrolyte concentrations of dialysate on LY conc. in permeate of binary HA/LY solution.	64
Fig. 4 31 Effect of different electrolyte concentrations of dialysate on reduce of LY conc of binary HA/LY solution.	65
Fig. 4 32 Removal of electrolyte from hyaluronic acid solution after diafiltration.	65
Fig. 4 33 Effect of different electrolytes & concentrations on the rejection of HA of binary HA/LY solution.	66
Fig. 4 34  LY conc. in permeate of binary HA/LY solution (1% g protein/g HA).	66
 
表目錄
Table 1 1 The classification of driving force in different operation process [2].	9
Table 3 1 The property of disc membrane	39
Table 3 2 The properties of hyaluronic acid	39
Table 3 3 The properties of lysozyme	39

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